Mouse Mouse IgG1 Isotype Control


SDS-Page: Mouse IgG1 Native Protein [NBP1-96983] - SDS-PAGE of Mouse IgG1 Kappa Isotype Control. Lane 1: Mouse IgG1 Kappa Isotype Control, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG1 Kappa Isotype more

Product Details

Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, IP, PAGE
1.0 mg/ml

Order Details

Mouse Mouse IgG1 Isotype Control Summary

A full-length protein corresponding to Mouse IgG1.

Source: Serum

Protein/Peptide Type
Native Protein
Antigen and protein A Affinity-purified


  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • SDS-Page 1:100 - 1:2000

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride pH 7.2
0.01% Sodium Azide
1.0 mg/ml
Antigen and protein A Affinity-purified


Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda. IgG1 is the most abundant of the four IgG subclasses. This isotype control possesses lambda light chains.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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