Mouse IgG, Fab2 Native Protein

Images

 
SDS-Page: Mouse IgG, Fab2 Native Protein [NBP1-97018] - SDS-PAGE of Mouse IgG F(ab')2 Fragment. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IP, PAGE
Concentration
LYOPH

Mouse IgG, Fab2 Native Protein Summary

Description
A full-length protein corresponding to Mouse IgG, Fab2.
Preparation
Method
Mouse IgG F(ab')2 fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(ab')2. No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
Protein/Peptide Type
Native Protein
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse IgG F(ab')2 fragment is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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