Mouse B Cell Isolation Kit

Images

 
Flow Cytometry: Mouse B Cell Isolation Kit [MAGM204-NOV] - Mouse splenocytes before (A) and after (B) isolation of B cells using the MagCellect. Histograms reflect staining of all viable cells with B220/CD45R-PE. Purity ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Kit Type
Isolation Kit

Order Details

Mouse B Cell Isolation Kit Summary

Description
The MagCellect Mouse B Cell Isolation Kit is designed to isolate B cells via a negative selection principle. The resulting cell preparation is highly enriched with B cells. Typical recovery ranges from 30-55% and the purity of recovered B cells ranges from 90-97%.

Kit Type
Isolation Kit

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1.0 ml of a Mouse B T Cell Biotinylated Antibody Cocktail
  2. 1.25 ml of a Streptavidin Ferrofluid
  3. 10 ml of a 10X Buffer

Background

MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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