Rat MMP-8 ELISA Kit (Colorimetric)

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ELISA: Rat MMP-8 ELISA Kit (Colorimetric) [KA1833] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum
Sensitivity
50 pg/mL

Order Details

Rat MMP-8 ELISA Kit (Colorimetric) Summary

Description
Mmp8 (Rat) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Mmp8.
Sensitivity
50 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MMP8

Applications/Dilutions

Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
  2. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution.
  3. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  4. Standards (Item C): 2 vials of recombinant rat MMP-8.
  5. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
  6. Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent.
  7. Detection Antibody MMP-8 (Item F): 2 vial of biotinylated anti-MMP-8 (each vial is enough to assay half microplate).
  8. HRP-Streptavidin Concentrate (Item G): 8 µl 8,000x concentrated HRP-conjugated streptavidin.
  9. MMP-8 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-Rat MMP-8.

Notes

Quality control test: Standard Curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rat MMP-8 ELISA Kit (Colorimetric)

  • CLG1HNC
  • Collagenase 2
  • EC 3.4.24
  • EC 3.4.24.34
  • matrix metallopeptidase 8 (neutrophil collagenase)
  • matrix metalloproteinase 8 (neutrophil collagenase)
  • Matrix metalloproteinase-8
  • MMP8
  • MMP-8
  • neutrophil collagenase
  • PMNL collagenase
  • PMNL-CL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MMP8
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