Human MMP-12 ELISA Kit (Colorimetric)

Images

 
ELISA: MMP-12 ELISA Kit [DDXK-E-MMP12-1] - Specificity of the ELISA for MMP-12. Detection of serial dilution of recombinant MMP-12 protein by the ELISA. (Black blocks) No recombinant MMP-1 (triangle), MMP-3 (upside down ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Human cell culture supernatants and biological fluids (plasma, serum, sputum).
Standard Curve Range
0.16 - 1 ug/ml
Sensitivity
The minimum detectable dose of human HME-MMP12 was determined to be <50pg/ml (Demets et al, 2006)

Order Details

Human MMP-12 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.16 - 1 ug/ml
Sensitivity
The minimum detectable dose of human HME-MMP12 was determined to be <50pg/ml (Demets et al, 2006)
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MMP12

Applications/Dilutions

Application Notes
The human HME-MMP12 ELISA kit is for quantitative determination of HME-MMP12 concentrations in human cell culture supernatants and biological fluids (plasma, serum, sputum). This kit is for research purpose only.
Publications
Read Publications using DDXK-E-MMP12-1.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Standard: Each vial contains a solution of recombinant HME-MMP12 (concentration indicated on the vial) produced, purified and concentrated from transfected eukaryotic cells. A 8 point standard curve using 2-fold serial dilutions in sample dilution buffer
  2. Capture Antibody: 0.5 mg/mL of mouse anti-HME-MMP12 monoclonal antibody (clone 705D10)
  3. Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-HME-MMP12 mouse monoclonal antibody (clone 706F9.01)

Notes

This product is manufactured by Dendritics and distributed by Novus Biologicals in North America and other select countries. To order in the following countries: Germany, Finland, United Kingdom, Norway, Denmark, Switzerland, Austria and South Korea, please contact Dendritics for additional ordering information.

Alternate Names for Human MMP-12 ELISA Kit (Colorimetric)

  • EC 3.4.24
  • hME
  • HMEEC 3.4.24.65
  • Macrophage elastase
  • macrophage metalloelastase
  • matrix metallopeptidase 12 (macrophage elastase)
  • matrix metalloproteinase 12 (macrophage elastase)
  • Matrix metalloproteinase-12
  • ME
  • MGC138506
  • MME
  • MMP12
  • MMP-12

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is critical for invasion and destruction in pathologies such as aneurysm and emphysema. HME mRNA and protein were previously described as expressed in human alveolar macrophages (Curci JA et al, 1998, J Clin Invest, 102(11):1900-10). The predicted molecular mass of the HME proenzyme is 54 kDa and readily undergoes NH2- and COOH-terminal processing to an active 45KDa form and a mature 22 kDa form. HME is a unique human metalloproteinase that displays elastolytic activity. We have isolated HME-MMP12 cDNA from a human CD34+-derived DC (GM-CSF+TNF) library. Expression analysis revealed HME-MMP12 expression in lung and placenta. Anti-HME-MMP12 monoclonal antibodies were obtained after mice immunization with HME-MMP12-transfected eukaryotic cells. These antibodies were used to set up a quantitative immunoassay for the detection of human HME-MMP12 (Demedts IK et al, 2006; Thorax, 61:196-201; Lapan et al, BMC Pulmonary Medicine, 2010, 10:40).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for MMP-12 ELISA Kit (DDXK-E-MMP12-1)(2)

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Product General Protocols

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Bioinformatics

Gene Symbol MMP12
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