MMP-12 Antibody

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Western Blot: MMP-12 Antibody [NBP1-52011] - Analysis of MMP-12 in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NBP1-52011 at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

MMP-12 Antibody Summary

Immunogen
Peptide with sequence C-RPKTSVNLISS corresponding to internal region according to NP_002417.2.
Epitope
C-RPKTSVNLISS
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP12
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 55 kDa band observed in human breast cancer lysates (calculated MW of 54.0 kDa band according to NP_002417.2).
Publications
Read Publication using NBP1-52011.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MMP-12 Antibody

  • EC 3.4.24
  • hME
  • HMEEC 3.4.24.65
  • Macrophage elastase
  • macrophage metalloelastase
  • matrix metallopeptidase 12 (macrophage elastase)
  • matrix metalloproteinase 12 (macrophage elastase)
  • Matrix metalloproteinase-12
  • ME
  • MGC138506
  • MME
  • MMP12
  • MMP-12

Background

Matrix metallopeptidase 12 (MMP-12) is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3); it contains a propeptide with autoinhibitory cysteine switch site, a well conserved zinc site, hinge region and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, and this breaks down to cascade of active forms, ending with the common 22 kDa form.Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-12 Antibody (NBP1-52011)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP12
Entrez
Uniprot