MKK3/MEK3 Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-41702
    • Availability
      Product Discontinued

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MKK3/MEK3 Antibody Summary

Immunogen
synthesized non-phosphopeptide derived from human MKK3 around the phosphorylation site of serine189 (V-D-SP-V-A).
Clonality
Polyclonal
Host
Rabbit
Gene
MAP2K3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100

Reactivity Notes

Human, Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MKK3/MEK3 Antibody

  • MAP kinase kinase 3
  • MAP2K3
  • MAPK/ERK kinase 3
  • MAPKK 3
  • MAPKK3PRKMK3dual specificity mitogen-activated protein kinase kinase 3
  • MEK3
  • MEK3MEK 3
  • mitogen activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 3
  • MKK3
  • MKK3EC 2.7.12.2
  • PRKMK3

Background

Mitogen-acitvated protein kinase kinase 3 (MKK3) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK3, closely related to MKK6, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MKK3 is activated by mitogenic and environ-mental stress stimulus as well as by insulin. MKK3 is necessary for the expression of glucose transporter and participates in the MAP kinase- mediated signaling cascade (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MAP2K3
Entrez