Mitotic Cells Antibody (8B3G) [PE]


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Product Details

Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Please see the vial label for concentration. If unlisted please contact technical services.

Mitotic Cells Antibody (8B3G) [PE] Summary

Clone 8B3G is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a total cell lysate of the human bladder carcinoma cell line T24.
8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorporation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle.
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
PE (A=488, E=575)
0.05% Sodium Azide
Protein A or G purified


  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence

Reactivity Notes



This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-97684APC
DyLight 350 Labeled NBP1-97684UV
DyLight 405 Labeled NBP1-97684V
DyLight 405LS Labeled NBP1-97684V3
DyLight 488 Labeled NBP1-97684G
DyLight 680 Labeled NBP1-97684FR
DyLight 755 Labeled NBP1-97684IR
PerCP Labeled NBP1-97684PCP

Additional Mitotic Cells Antibody (8B3G) [PE] Products

Mitotic Cells NBP1-97684PE

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