Mitochondria Antibody (113-1) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clone
113-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Mitochondria Antibody (113-1) [Janelia Fluor® 646] Summary

Immunogen
Semi-purified mitochondrial preparation
Localization
Mitochondria in cytoplasm
Marker
Marker for Human Cells, Granular RCC & Salivary Tumors
Specificity
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Mitochondria Antibody (NBP2-34517JF646) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-34517AF405
Alexa Fluor 488 NBP2-34517AF488
Alexa Fluor 647 NBP2-34517AF647
Alexa Fluor 700 NBP2-34517AF700
Biotin NBP2-34517B
DyLight 350 NBP2-34517UV
DyLight 405 NBP2-34517V
DyLight 488 NBP2-34517G
DyLight 550 NBP2-34517R
DyLight 650 NBP2-34517C
DyLight 680 NBP2-34517FR
DyLight 755 NBP2-34517IR
FITC NBP2-34517F
HRP NBP2-34517H
Janelia Fluor 549 NBP2-34517JF549
Janelia Fluor 646 NBP2-34517JF646

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Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

The Importance of the COX IV Antibody to Apoptosis Research
COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading...  Read full blog post.

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