Mitochondria Antibody (113-1)

Images

 
Western Blot: Mitochondria Antibody (113-1) [NBP2-32980] - Western Blot analysis of HeLa cell lysate using Mitochondria Antibody (113-1).
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517] - Formalin-fixed paraffin-embedded human Renal ...read more
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Analysis using the Biotin conjugate of NBP2-32980. Staining of human prostate cancer tissue (left) and mouse stomach tissue (right). Image from ...read more
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with mitochondria monoclonal antibody (113-1). Image using the Azide and ...read more
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Formalin-fixed paraffin-embedded human pancreas stained with mitochondria monoclonal antibody (113-1). Image using the Azide and BSA Free form ...read more
Immunofluorescence: Mitochondria Antibody (113-1) [NBP2-32980] - Confocal immunofluorescence image of HeLa cells using Mitochondria Antibody (113-1) labeled is Green (CF488) and Red Dot is used to label the nuclei Red.

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC/IF
Clone
113-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Mitochondria Antibody (113-1) Summary

Immunogen
Semi-purified mitochondrial preparation
Localization
Mitochondria in cytoplasm
Marker
Marker for Human Cells, Granular RCC & Salivary Tumors
Specificity
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5ug/ml
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • Immunofluorescence
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mitochondria Antibody (NBP2-32980) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mitochondria Antibody (NBP2-32980) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-34517AF405
Alexa Fluor 488 NBP2-34517AF488
Alexa Fluor 647 NBP2-34517AF647
Alexa Fluor 700 NBP2-34517AF700
Biotin NBP2-34517B
DyLight 350 NBP2-34517UV
DyLight 405 NBP2-34517V
DyLight 488 NBP2-34517G
DyLight 550 NBP2-34517R
DyLight 650 NBP2-34517C
DyLight 680 NBP2-34517FR
DyLight 755 NBP2-34517IR
FITC NBP2-34517F
HRP NBP2-34517H
Janelia Fluor 549 NBP2-34517JF549
Janelia Fluor 646 NBP2-34517JF646

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Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

The Importance of the COX IV Antibody to Apoptosis Research
COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading...  Read full blog post.

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