Recombinant MIF Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43181
    • Availability
      Product Discontinued

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Recombinant MIF Protein Summary

Description
A partial protein corresponding to MIF.
Preparation
Method
E. Coli.
Details of Functionality
The optimal range of activity found in an agarose microdroplet assay using human U937 cells is 0.25-1ug/ml.
Protein/Peptide Type
Partial Recombinant Protein
Gene
MIF
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
This reagent has been tested in an agarose microdroplet assay. The optimal range of activity measured by percentage of migration inhibition on U937 cells was 0.25-1ug/ml.

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 97%, as determined by SDS-PAGE

Alternate Names for Recombinant MIF Protein

  • EC 5.3.2.1
  • EC 5.3.3.12
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MIF
  • MMIF
  • Phenylpyruvate tautomerase

Background

MIF (migration inhibitory factor, or macrophage migration inhibitory factor, MMIF) is an inflammatory mediator in response to bacterial lipopolysaccharide. MIF stimulates IL-1, IL-8 and MMP expression on fibroblasts. MIF stimulates NO and TNF-alpha production on macrophages. The secretion of MIF is regulated by steroids and MIF counter-regulates the immunosuppressive effects of steroids on immune response and cytokine induction. Therefore MIF plays an important role in endotoxemia and septic shock. The expression of MIF activity is correlated with delayed hypersensitivity and cellular immunity. It has been reported that the expression of MIF is significantly increased in cardiomyocytes in chronic hypoxia and the expression of MIF is implicated in cancer pathogenesis. Human MIF is a non-glycosylated polypeptide consisting of 115 amino acids with a molecular weight of 12.5 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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NBP1-43181
Applications: IA

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Bioinformatics

Gene Symbol MIF
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