MHC Class I Antibody (SF1-1.1.1) [Biotin]

Images

 
Flow Cytometry: MHC Class I Antibody (SF1-1.1.1) [Biotin] [NBP1-43514] - Staining of BALB/c (left) and C57BL/6 (right) splenocytes with 0.125 micrograms of Mouse IgG2a k Isotype Control Biotin (blue histogram) or 0.125 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
SF1-1.1.1
Clonality
Monoclonal
Host
Mouse
Concentration
0.5 mg/ml

Order Details

MHC Class I Antibody (SF1-1.1.1) [Biotin] Summary

Immunogen
The immunogen for this antibody was MHC Class I.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HLA-E
Purity
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Biotin
Format
Biotin (A=N/A, E=N/A)
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This SF1-1.1.1 antibody has been tested by flow cytometric analysis on Balb/c splenocytes. This antibody can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Readout System

Reactivity Notes

This antibody is reactive to Mouse.

Alternate Names for MHC Class I Antibody (SF1-1.1.1) [Biotin]

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1

Background

This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HLA-E

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