Methylated Lysine Antibody [Biotin]

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Product Details

Summary
Product Discontinued
View other related Methylated Lysine Primary Antibodies

Order Details


    • Catalog Number
      NB110-96899
    • Availability
      Product Discontinued

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Methylated Lysine Antibody [Biotin] Summary

Immunogen
Methylated KLH Conjugated detects proteins containing methylated lysine residues in SDS PAGE immunoblots.
Specificity
Detects proteins containing methylated lysine residues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:5000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunoprecipitation 1:10-1:500
Application Notes
0.2-0.5 ug/ml of Methylated Lysine Antibody : Biotinwas sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Methylated Lysine Antibody [Biotin]

  • Dimethylysine
  • Methyl lysine
  • N epsilon dimethyl lysine
  • N epsilon mono methyllysine
  • N epsilon trimethyl lysine
  • N(epsilon) trimethyl lysine
  • Trimethyl lysine

Background

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Methylated Lysine Products

Methylated Lysine NB110-96899

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