metabotropic Glutamate Receptor 1a Antibody

Images

 
Western Blot: metabotropic Glutamate Receptor 1a Antibody [NB110-39033] - WB detection of GluR1 in 20 ug of human brain lysate (lane 2) with GluR1 polyclonal antibody at 1:1000 dilution followed by AP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: metabotropic Glutamate Receptor 1a Antibody [NB110-39033] - Primary Rat Cortical Neurons, image courtesy of customer.
Immunohistochemistry-Frozen: metabotropic Glutamate Receptor 1a Antibody [NB110-39033] - Immunofluorescence labeling for GluR1 in rat brain (cingulate cortex). GluR1 detection was done using anti-rabbit Cy3 conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

metabotropic Glutamate Receptor 1a Antibody Summary

Immunogen
Synthetic peptide comprising residues 1159-1171 [SSVPSSPVSESVL] of the human mGluR1 protein.
Localization
Cell membrane
Specificity
Glutamate Receptor
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
This metabotropic Glutamate Receptor 1a antibody is useful for Western Blot, Immunohistochemistry-Frozen and Immunohistochemistry-paraffin applications. Use in Immunocytochemistry/immunofluorescence was reported in scientific literature (PMID: 19688350) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
132.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NMDAR1 Lysate (NBL1-11338)
Reviewed Applications
Read 1 Review rated 4
using
NB110-39033 in the following applications:

Publications
Read Publication using
NB110-39033 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for metabotropic Glutamate Receptor 1a Antibody

  • glutamate receptor, metabotropic 1
  • GRM1A
  • metabotropic glutamate receptor 1
  • mGluR1
  • MGLUR1A
  • MGLUR1GPRC1AmGlu1

Background

The Metabotropic Glutamate Receptor mGluR1 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. mGluR1 and mGluR5 are group I receptors that have been shown to activate phospholipase C. Alternative splice variants of the mGluR1 gene have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain. mGluR1 has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries. Caution: GRIA1 refers to Ionotropic Glutamate Receptor 1, not to Metabotropic Glutamate Receptor 1 (mGLUR1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for metabotropic Glutamate Receptor 1a Antibody (NB110-39033)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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Review for metabotropic Glutamate Receptor 1a Antibody (NB110-39033) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: ICC.

Reviews using NB110-39033:
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ICC
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry metabotropic Glutamate Receptor 1a NB110-39033
Enlarge
reviewed by:
Janosch Heller
ICC Rat 03/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary rat cortical neurons
SpeciesRat

Comments

CommentsFixation: 4% paraformaldehyde (TAAB) in PBS, 15 minutes RT
Quenching: 0.1% NaBH4 in PBS, 7 minutes RT
Blocking: 3% BSA in PBS, 30 minutes RT
Permeabilization: 0.1% Triton-X100, 10 minutes RT
Primary Antibody: 1:500 in 3% BSA in PBS, overnight, 4°C
Wash: three times wash with PBS, 10 min each
Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:1800 in PBS with 1% BSA, 2 hours, RT
Wash: three times wash with PBS, 10 min each
Microscope: Bruker Vutara 350

The antibody labels clusters of metabotropic receptors along the whole cell.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for metabotropic Glutamate Receptor 1a Antibody (NB110-39033) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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metabotropic Glutamate Receptor 1a NB110-39033

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Janosch Heller
03/27/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol GRM1
Entrez
Uniprot