Mesothelin Antibody

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Western Blot: Mesothelin Antibody [NBP1-51998] - (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Mesothelin Antibody Summary

Immunogen
Peptide with sequence CPSGKKAREIDES corresponding to internal region according to NP_005814.2, NP_037536.2.
Epitope
CPSGKKAREIDES
Specificity
This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MSLN
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 70 kDa band observed in human placenta lysates (calculated MW of 68.1 kDa band according to NP_005814.2 and of 69.0 kDa band according to NP_037536.2). An additional band of unknown identity was also consistently observed at 150 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-51998.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mesothelin Antibody

  • CAK1 antigen
  • CAK1
  • megakaryocyte potentiating factor
  • Mesothelin
  • MPF
  • MPFSMRP
  • MSLN
  • Pre-pro-megakaryocyte-potentiating factor
  • SMR
  • soluble MPF mesothelin related protein

Background

An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mesothelin Antibody (NBP1-51998)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MSLN
Entrez
Uniprot