Western Blot: Melan-A/MART-1 Antibody (MLANA/788) [NBP2-44394] - Western Blot Analysis of COLO 38 cell lysate using Melan-A/MART-1 Mouse Monoclonal Antibody (MLANA/788).
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (MLANA/788) [NBP2-44394] - Human Testis stained with Melan-A Monoclonal Antibody (MLANA/788).
Flow Cytometry: Melan-A/MART-1 Antibody (MLANA/788) [NBP2-44394] - Flow Cytometry: Melan-A/MART-1 Antibody (MLANA/788) [FITC] [NBP2-47803F] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 ...read more
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (MLANA/788) [NBP2-44394] - Human Melanoma stained with Melan-A Monoclonal Antibody (MLANA/788).
Recombinant human full-length Melan-A/MART-1 protein
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Protein A or G purified
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Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Use in Immunohistochemistry reported in scientific literature (PMID: 30606914).
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