Melan-A/MART-1 Antibody (MLANA/788)

Images

 
Western Blot: Melan-A/MART-1 Antibody (MLANA/788) [NBP2-44394] - Western Blot Analysis of COLO 38 cell lysate using Melan-A/MART-1 Mouse Monoclonal Antibody (MLANA/788).
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (MLANA/788) [NBP2-44394] - Human Testis stained with Melan-A Monoclonal Antibody (MLANA/788).
Flow Cytometry: Melan-A/MART-1 Antibody (MLANA/788) [NBP2-44394] - Flow Cytometry: Melan-A/MART-1 Antibody (MLANA/788) [FITC] [NBP2-47803F] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 ...read more
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (MLANA/788) [NBP2-44394] - Human Melanoma stained with Melan-A Monoclonal Antibody (MLANA/788).

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
MLANA/788
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Melan-A/MART-1 Antibody (MLANA/788) Summary

Immunogen
Recombinant human full-length Melan-A/MART-1 protein
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLANA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Immunohistochemistry reported in scientific literature (PMID: 30606914).
Publications
Read Publication using
NBP2-44394 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-47803).

Alternate Names for Melan-A/MART-1 Antibody (MLANA/788)

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • Melan-A
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melan-A/MART-1 Antibody (NBP2-44394)(1)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Melan-A/MART-1 Antibody (NBP2-44394) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47803AF350
Alexa Fluor 405 NBP2-47803AF405
Alexa Fluor 488 NBP2-47803AF488
Alexa Fluor 532 NBP2-47803AF532
Alexa Fluor 594 NBP2-47803AF594
Alexa Fluor 647 NBP2-47803AF647
Alexa Fluor 700 NBP2-47803AF700
Alexa Fluor 750 NBP2-47803AF750
Allophycocyanin NBP2-47803APC
Allophycocyanin/Cy7 NBP2-47803APCCY7
Biotin NBP2-47803B
DyLight 350 NBP2-47803UV
DyLight 405 NBP2-47803V
DyLight 488 NBP2-47803G
DyLight 550 NBP2-47803R
DyLight 594 NBP2-47803DL594
DyLight 650 NBP2-47803C
DyLight 680 NBP2-47803FR
DyLight 755 NBP2-47803IR
FITC NBP2-47803F
HRP NBP2-47803H
Janelia Fluor 549 NBP2-47803JF549
Janelia Fluor 646 NBP2-47803JF646
PE NBP2-47803PE
PE/Atto594 NBP2-47803PEATT594
PE/Cy5.5 NBP2-47803PECY55
PE/Cy7 NBP2-47803PECY7
PerCP NBP2-47803PCP

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Bioinformatics

Gene Symbol MLANA
Uniprot