MEKK3 Antibody

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Western Blot: MEKK3 Antibody [NBP1-71888] - Whole cell lysate from 293T (5, 15 and 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.4 ug/ml.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-71888
    • Availability
      Product Discontinued

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MEKK3 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 125 and 175 of human MAP/ERK Kinase Kinase 3 using the numbering given in entry NP_002392.2 (GeneID 4215).
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MEKK3 Antibody

  • EC 2.7.11.25
  • MAP/ERK kinase kinase 3
  • MAP3K3
  • MAPK/ERK kinase kinase 3
  • MAPKKK3
  • MAPKKK3MEKK 3
  • MEK kinase 3
  • MEKK3
  • mitogen-activated protein kinase kinase kinase 3

Background

MEKK3 is a Ser/Thr protein kinase belonging to the MAP/ERK kinase kinase family (1). Closely related to MEKK2, MEKK3 is expressed in a multitude of tissues. MEKK3 directly regulates SAPKs and ERKs pathway via SEK and MEK1, respectively but not the p38 pathway (2) and is also involved in the NF-kB pathway (3). MEKK3 as well as MEKK2 bind, phosphorylate and activate MEK5 through their PB1 domain (4). hKSR-2 (human kinase suppressor of ras-2) has been shown to regulate the activity of MEKK3 (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP3K3
Entrez
Uniprot