MEK2 Antibody (SD207-08)

Western Blot: MEK2 Antibody (SD207-08) [NBP2-67791] - Analysis of MEK2 on different lysates using anti-MEK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: 293T

Immunocytochemistry/Immunofluorescence: MEK2 Antibody (SD207-08) [NBP2-67791] - ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MEK2 Antibody (SD207-08) [NBP2-67791] - Analysis of paraffin-embedded mouse lung tissue using anti-MEK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: MEK2 Antibody (SD207-08) [NBP2-67791] - Analysis of Hela cells with MEK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: MEK2 Antibody (SD207-08) [NBP2-67791] - Staining MEK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MEK2 Antibody (SD207-08) [NBP2-67791] - Staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MEK2 Antibody (SD207-08) [NBP2-67791] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MEK2 Antibody (SD207-08) [NBP2-67791] - Analysis of paraffin-embedded human liver cancer tissue using anti-MEK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MEK2 Antibody (SD207-08) [NBP2-67791] - Analysis of paraffin-embedded human spleen tissue using anti-MEK2 antibody. Counter stained with hematoxylin.

Product Discontinued

MEK2 Antibody (SD207-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human MEK2 aa 1-50/400. (SwissProt: P36507 Human; SwissProt: Q63932 Mouse)
• Localization: Cytoplasm, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP2K2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MEK2 Antibody (SD207-08)

• ERK activator kinase 2
• MAP2K2
• MAPKK 2
• MEK 2
• MEK2
• MEK2EC 2.7.12.2
• mitogen-activated protein kinase kinase 2
• MKK2
• MKK2FLJ26075
• p45
• PRKMK2MAPKK2

Background

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K2