MED7 Overexpression Lysate (Denatured)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MED7 Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-CRSP9 full-length
Gene
MED7

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for MED7

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-CRSP9 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED7 Overexpression Lysate (Denatured)

  • Activator-recruited cofactor 34 kDa component
  • Cofactor required for Sp1 transcriptional activation subunit 9
  • cofactor required for Sp1 transcriptional activation, subunit 9 (33kD)
  • cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
  • CRSP33CRSP complex subunit 9
  • CRSP9hMED7
  • mediator complex subunit 7ARC34
  • mediator of RNA polymerase II transcription subunit 7
  • MGC12284
  • RNA polymerase transcriptional regulation mediator subunit 7 homolog
  • Transcriptional coactivator CRSP33

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol MED7