MED27 Overexpression Lysate (Denatured)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MED27 Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-CRSP8 full-length
Gene
MED27

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for MED27

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-CRSP8 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED27 Overexpression Lysate (Denatured)

  • cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa
  • CRSP34CRSP complex subunit 8
  • CRSP8CRAP34
  • mediator complex subunit 27Transcriptional coactivator CRSP34
  • mediator of RNA polymerase II transcription subunit 27
  • MGC11274
  • P37 TRAP/SMCC/PC2 subunit
  • subunit 8 (34kD)
  • TRAP37

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics Tool for MED27 Lysate (H00009442-T01)

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Bioinformatics

Gene Symbol MED27