MED23 Antibody

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Product Details

Summary
Product Discontinued
View other related MED23 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-11244
    • Availability
      Product Discontinued

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MED23 Antibody Summary

Immunogen
Synthetic 24mer peptide corresponding to unique epitope on MED23 protein taken from amino acids 650-716. The peptide sequence was unique to MED23 protein and was not present on any other glutamate receptor family members. The MED23 peptide was covalently modified post-synthetically covalently modified to achieve desired antigenicity.
Specificity
These antibodies do not cross react to other members of the transcription factor family or to other receptor interacting proteins.
This antibody detects a band of approximately 133 kDa in PC-MED23 samples and in several cell lines
Clonality
Polyclonal
Host
Rabbit
Gene
MED23
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:10000
Application Notes
It is ideal for WB and ELISA applications, other applications have not been tested

Reactivity Notes

Human, Mouse and Rat

Alternate Names for MED23 Antibody

  • 130 kDa transcriptional co-activator
  • 133 kDa transcriptional co-activator
  • CRSP130
  • DRIP130DKFZp434H0117
  • mediator complex subunit 23CRSP133
  • Protein sur-2 homolog
  • subunit 3, 130kDa
  • Transcriptional coactivator CRSP130
  • Vitamin D3 receptor-interacting protein complex 130 kDa component

Background

Gene transcription is a multistep process that is triggered by transcription factors. The Mediator Complex is a multi-protein complex that acts as a molecular bridge linking transcription factors that are gene-specific to the basal transcription machinery. This bridge is connected through direct interactions with proteins; linking DNA bound transcription factors and RNA polymerase II (Pol II). Mediator subunits control different genes and physiological processes, depending on the different transcription factors that interact with environmental stimuli. An active mediator complex has been found to be the size of a ribosomal subunit (1). This surface area is large enough to potentially act as a site for a multitude of different transcriptional regulators having influence on transcription initiation. The Mediator Complex supports the transcription of genes, holding diverse transcription factor initiation sites (2). MED23 cells-also known as Mediator of RNA polymerase II transcription subunit 23 contains an active mediator complex that supports the transcription of most genes (1). The mammalian mediator subunit MED23 is key for the Erg1 (early growth response factor 1) transcription factor in mouse embryonic stem cells (2). The Erg1 transcription factor plays a role in cell growth regulation, cell differentiation, cell transformation and apoptosis (2). The mediator MED23 subunit has also been found to have a correlation with the ternary complex factor Elk1. Transcript elongation that is made via Pol II is paused at the Erg1 promoter-which is activated via MAPK phosphorylation of Elk1 and the phospho-Elk1 interactions with the mediator occurring through the MED23 subunit (2). The MED23 gene is expressed in multiple spliced variant forms. MED23-selective antibodies were generated against unique antigenic peptide taken form the region between amino acids 650-700 in the MED23 protein, the selected peptide sequence is present in all the variants of MED23 but not found in any other protein listed in the gene bank.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Contact Information

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Bioinformatics

Gene Symbol MED23

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