MDM2/HDM2 Antibody

Images

 
Western Blot: MDM2/HDM2 Antibody [NB100-2827] - Analysis of MDM2/HDM2(isoform) using this antibody at 0.1 ug/ml in Human Liver lysate (35ug protein in RIPA buffer).
Western Blot: MDM2/HDM2 Antibody [NB100-2827] - HEK293 overexpressing MDM2/HDM2 and probed with (mock transfection in first lane).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MDM2/HDM2 Antibody Summary

Immunogen
Peptide with sequence C-DELSGERQRKRHKSD corresponding to internal region according to NP_002383.2.
Specificity
This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MDM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 56.0 kDa band according to NP_002383.2). In transfected HEK293 transiently expressing MDM2 bands of approx. 48 kDa, 60 kDa, 70 kDa, and 90 kDa band are observed. These bands are not observed in the non-transfected HEK293. The 80 kDa band is endogenously expressed in the negative control.
Positive Control
Liver Lysate (NB820-59232)
MDM2/HDM2 Lysate (NBL1-12970)
Publications
Read Publication using NB100-2827.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Feline, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDM2/HDM2 Antibody

  • ACTFS
  • HDM2
  • HDMX
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • MDM2

Background

MDM2 is an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 transcription factor. MDM2 is a p53 responsive gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDM2/HDM2 Antibody (NB100-2827)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MDM2
Entrez
Uniprot