MD-2 Antibody (9F1B1)

Images

 
Western Blot: MD-2 Antibody (9F1B1) [NBP1-75512] - analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: MD-2 Antibody (9F1B1) [NBP1-75512] - MD2 in human spleen tissue with MD2 antibody at 20 ug/mL.
Immunohistochemistry: MD-2 Antibody (9F1B1) [NBP1-75512] - MD-2 in human spleen with MD-2 antibody at 2.5 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
9F1B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MD-2 Antibody (9F1B1) Summary

Immunogen
Antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LY96
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
MD-2 antibody can be used for detection of MD-2 by Western blot at 0.5 -1 ug/mL.

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MD-2 Antibody (9F1B1)

  • ESOP1
  • ESOP-1
  • LY96
  • ly-96
  • lymphocyte antigen 96
  • MD2
  • MD-2
  • myeloid differentiation protein-2
  • Protein MD-2

Background

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LY96
Entrez
Uniprot