MCM4 Antibody

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Western Blot: MCM4 Antibody [NBP1-58072] - Jurkat cell lysate, concentration 2.5 ug/ml.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-58072
    • Availability
      Product Discontinued

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MCM4 Antibody Summary

Immunogen
Synthetic peptides corresponding to MCM4 (minichromosome maintenance complex component 4) The peptide sequence was selected from the N terminal of MCM4. Peptide sequence SRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSL.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCM4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against MCM4 and was validated on Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for MCM4 Antibody

  • CDC21 homolog
  • CDC21
  • CDC54
  • DNA replication licensing factor MCM4
  • EC 3.6.4.12
  • hCdc21
  • homolog of S. pombe cell devision cycle 21
  • MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)
  • MCM4 minichromosome maintenance deficient 4
  • MGC33310
  • minichromosome maintenance complex component 4
  • minichromosome maintenance deficient 4
  • P1-Cdc21

Background

The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MCM4
Uniprot