mCherry Antibody

Images

 
Western Blot: mCherry Antibody [NBP2-25158] - Blot of Hek293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30kDa. ...read more
Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25158] - HeK293 cells transfected in the same way and viewed in the confocal microscope. Most Hek293 cells are not transfected so only the nucleus of these ...read more

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

mCherry Antibody Summary

Immunogen
Full length recombinant protein in Chicken
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
Application Notes
This mCherry antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~30 kDa. Use in Immunohistochemistry reported in scientific literature (PMID 27396338).
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-25158 in the following applications:

Publications
Read Publications using
NBP2-25158 in the following applications:

Reactivity Notes

This antibody detects mCherry.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Antibody is supplied as an aliquot of concentrated IgY prep (chicken egg)
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for mCherry Antibody

  • DSRED
  • red fluorescent protein mCherry
  • Red Fluoroscent Protein

Background

mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (NBP2-25158)(4)

We have publications tested in 2 confirmed species: Mouse, Monkey.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


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ICC/IF
(1)
IHC
(2)
IHC-P
(1)
All Applications
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Mouse
(1)
Monkey
(1)
All Species

Review for mCherry Antibody (NBP2-25158) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Drosophila.

Reviews using NBP2-25158:
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Filter by Species
Drosophila
(1)
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Images Ratings Applications Species Date Details
 mCherry NBP2-25158
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reviewed by:
Masahiko Takemura
Drosophila 12/08/2016
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Summary

Sample TestedWing imaginal disc
SpeciesDrosophila

Comments

Comments1:1000 dilution

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mCherry Antibody (NBP2-25158). (Showing 1 - 1 of 1 FAQs).

  1. What isotype is this anti mcherry antibody NBP2-25158?
    • IGY

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Recent Reviews

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Masahiko Takemura
12/08/2016
Application:
Species: Drosophila