Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these ...read more
Genetic Strategies: Western Blot: mCherry Antibody [NBP2-25157] - 3 cells transfected with pFin-EF1-mCherry vector, in the lane marked '+'. HEK293 cells which were not transfected with this vector show no protein ...read more
This mCherry antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~30 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID 27396338). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28891816).
30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 3 Reviews rated 5 using NBP2-25157 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM Sodium Azide
Immunogen affinity purified
Alternate Names for mCherry Antibody
red fluorescent protein mCherry
Red Fluoroscent Protein
mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for mCherry Antibody (NBP2-25157)(9)
We have publications tested in 4 confirmed species: Human, Mouse, Rat, Invertebrate.
We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.
FAQs for mCherry Antibody (NBP2-25157). (Showing 1 - 2 of 2 FAQs).
Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
I was wondering if you had any literature on whether the polyclonal mCherry antibody NBP2-25157 shows cross-reaction with td-tomato?
We do see 88% homology between the mCherry and the dTomato sequences and therefore, the antibody will likely see both of these RFP proteins. The antibody is a polycloanl which then increases the likelihood that it will have multiple binding sites along the homologous sequences. There really is no regions of sequence where one could make an antibody that did not see both proteins.
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