mCherry Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these ...read more
Genetic Strategies: Western Blot: mCherry Antibody [NBP2-25157] - 3 cells transfected with pFin-EF1-mCherry vector, in the lane marked '+'. HEK293 cells which were not transfected with this vector show no protein ...read more

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

mCherry Antibody Summary

Immunogen
Recombinant full length His tagged mCherry purified from E. coli
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin
Application Notes
This mCherry antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~30 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID 27396338). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28891816).
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 5
using
NBP2-25157 in the following applications:

Publications
Read Publications using
NBP2-25157 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
10mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for mCherry Antibody

  • DSRED
  • red fluorescent protein mCherry
  • Red Fluoroscent Protein

Background

mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (NBP2-25157)(14)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Drosophila, Invertebrate.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(6)
IHC
(2)
IHC-P
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(2)
Drosophila
(2)
Invertebrate
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP2-25157 Applications Species
Lui S, Torontali Z, Tadjalli A et al. Brainstem Nuclei Associated with Mediating Apnea-Induced Respiratory Motor Plasticity Sci Rep Aug 23 2018 [PMID: 30139983] (ICC/IF, Rat) ICC/IF Rat
Carreira-Rosario A, Zarin AA, Clark MQ et al. MDN brain descending neurons coordinately activate backward and inhibit forward locomotion Elife Aug 2 2018 [PMID: 30070205] (ICC/IF, Drosophila) ICC/IF Drosophila
Seo JH, Agarwal E, Bryant KG et al. Syntaphilin Ubiquitination Regulates Mitochondrial Dynamics And Tumor Cell Movements Cancer Res. Jun 13 2018 [PMID: 29898993] (IHC-P, Mouse) IHC-P Mouse
Jeong JH, Lee DK, Liu SM et al. Activation of temperature-sensitive TRPV1-like receptors in ARC POMC neurons reduces food intake PLoS Biol. Apr 1 2018 [PMID: 29689050] (ICC/IF, Mouse) ICC/IF Mouse
Hinaux H, Bachem K, Battistara M et al. Revisiting the developmental and cellular role of the pigmentation gene yellow in Drosophila using a tagged allele. Dev. Biol. Apr 7 2018 [PMID: 29634916] (Drosophila) Drosophila
Xu X, Iwasa H, Hossain S et al. BCL-XL binds and antagonizes RASSF6 tumor suppressor to suppress p53 expression Genes Cells. 2017 Nov 28 [PMID: 29193479] (Invertebrate) Invertebrate
Caino MC, Seo JH, Wang Y et al. Syntaphilin controls a mitochondrial rheostat for proliferation-motility decisions in cancer J. Clin. Invest. 2017 Sep 11 [PMID: 28891816] (IHC-P, Mouse) IHC-P Mouse
Lee I, Kim GS, Bae JS et al. The DNA replication protein Cdc6 inhibits the microtubule-organizing activity of the centrosome J. Biol. Chem. 2017 Aug 21 [PMID: 28827311] (ICC/IF, Human) ICC/IF Human
Takemura M, Nakato H. Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration. J. Cell. Sci. 2016 Nov 25 [PMID: 27888216] (IHC) IHC
Cho A, Kato M, Whitwam T et al. An Atypical Tropomyosin in Drosophila with Intermediate Filament-like Properties Cell Rep 2016 Jul 26 [PMID: 27396338] (IHC) IHC
Show All 14 Publications.

Reviews for mCherry Antibody (NBP2-25157) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Drosophila.

Reviews using NBP2-25157:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Drosophila
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot mCherry NBP2-25157
Enlarge
reviewed by:
Anonymous
WB Human 08/22/2017
View

Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate
SpeciesHuman
Lot012016
 mCherry NBP2-25157
Enlarge
reviewed by:
Masahiko Takemura
Drosophila 12/08/2016
View

Summary

Sample TestedWing imaginal disc
SpeciesDrosophila
Lot22614

Comments

Comments1:1000 dilution
Immunofluorescence mCherry NBP2-25157
Enlarge
reviewed by:
Amanpreet Kaur
IF Mouse 07/23/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse melanoma with mCherry overexpression
SpeciesMouse
Lot22614

Blocking

Blocking DetailsHydrogen peroxide block for 20 minutes (Ultravision)

Primary Anitbody

Dilution Ratio1:500 at 4 degrees Celsius overnight. Antibody was diluted in Antibody Diluent (Dako)

Secondary Antibody

Secondary DescriptionGoat anti-rabbit (Ultravision)
Secondary Manufacturer Cat#TR-060-BN (Fisher)

Details

Detection NotesAEC Chromogen - #TA060SA
Fixation DetailsFixation -10% formalin, antigen retrival - citrate buffer followed by steaming for 20 minutes.
Wash Description1x PBS for 5 minutes 3 times.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mCherry Antibody (NBP2-25157). (Showing 1 - 2 of 2 FAQs).

  1. Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
    • mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
  2. I was wondering if you had any literature on whether the polyclonal mCherry antibody NBP2-25157 shows cross-reaction with td-tomato?
    • We do see 88% homology between the mCherry and the dTomato sequences and therefore, the antibody will likely see both of these RFP proteins.   The antibody is a polycloanl which then increases the likelihood that it will have multiple binding sites along the homologous sequences.    There really is no regions of sequence where one could make an antibody that did not see both proteins.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anonymous
08/22/2017
Application: WB
Species: Human

Masahiko Takemura
12/08/2016
Application:
Species: Drosophila

Amanpreet Kaur
07/23/2015
Application: IF
Species: Mouse