MBD3 Antibody

Images

 
Western Blot: MBD3 Antibody [NBP1-40346] - analysis of MBD3 in Jurkat cell lysate (30ug) using anti-MBD3 antibody followed by goat anti-rabbit HRP conjugated secondary antibody (Cat. #NB7160). Image from verified ...read more
Immunoprecipitation: MBD3 Antibody [NBP1-40346] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-MBD3 antibody used for IP at 10 mcg/mg lysate. MBD3 was also ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MBD3 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 230 and 265 of human methyl-CpG binding domain protein 3 using the numbering given in entry NP_003917.1 (GeneID 53615).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MBD3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunoprecipitation 5-15 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MBD3 Antibody

  • MBD3
  • methyl-CpG binding domain protein 3
  • methyl-CpG-binding domain protein 3
  • Methyl-CpG-binding protein MBD3

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MBD3
Entrez