MBD2 Antibody

Images

 
Western Blot: MBD2 Antibody [NBP1-52894] - Lanes: Lane 1: 15 ug WT mouse ES lysate Lane 2: 15 ug MBD2 KO mouse ES lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Goat anti-rabbit-HRP Secondary Antibody ...read more
Immunohistochemistry: MBD2 Antibody [NBP1-52894] - Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Nucleus Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: ...read more
Western Blot: MBD2 Antibody [NBP1-52894] - HepG2 cell lysate, concentration 0.625ug/ml.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-52894
    • Availability
      Product Discontinued

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MBD2 Antibody Summary

Immunogen
Synthetic peptides corresponding to MBD2 (methyl-CpG binding domain protein 2) The peptide sequence was selected from the middle region of MBD2. Peptide sequence DCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNT.
Clonality
Polyclonal
Host
Rabbit
Gene
MBD2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry
Application Notes
This is a rabbit polyclonal antibody against MBD2 and was validated on Western blot.
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for MBD2 Antibody

  • demethylase
  • DKFZp586O0821
  • DMTase
  • methyl-CpG binding domain protein 2
  • methyl-CpG-binding domain protein 2
  • Methyl-CpG-binding protein MBD2
  • NY-CO-41

Background

MBD2 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD2 can also repress transcription from methylated gene promoters. MBD2 may function as a mediator of the biological consequences of the methylation signal.It is also reported that the MBD2 functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MBD2
Uniprot