MARK1/2/3/4 Antibody

Images

 
Western Blot: MARK1/2/3/4 Antibody [NBP1-00910] - Analysis of MARK1/2/3/4 (N211) pAb in extracts from A549 cells.
Immunohistochemistry-Paraffin: MARK1/2/3/4 Antibody [NBP1-00910] - Analyzes of MARK1/2/3/4 (N211) pAb in paraffin-embedded human breast carcinoma tissue.
Western Blot: MARK1/2/3/4 Antibody [NBP1-00910] - Analysis of MARK1/2/3/4 (N211) antibody in extracts from COS7 cells

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00910
    • Availability
      Product Discontinued

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MARK1/2/3/4 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 180-230 of Human MARK1.
Clonality
Polyclonal
Host
Rabbit
Gene
MARK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of MARK1/2/3/4 (N211) pAb in extracts from A549 cells.
Immunohistochemistry (IHC) analyzes of MARK1/2/3/4 (N211) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MARK1/2/3/4 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA1477serine/threonine-protein kinase MARK1
  • MAP/microtubule affinity-regulating kinase 1MGC126512
  • MARKMGC126513

Background

MARK1 (MAP/microtubule affinity-regulating kinase 1) belongs to the MARK family of serine/threonine kinases. MARK family protein kinases phosphorylate microtubule-associated proteins and may play a role in cytoskeletal stability. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. MARK3 was originally identified as a marker that was induced by treatment with DNA damaging agents, and loss of MARK3 was found with carcinogenesis in the pancreas. MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MARK1
Entrez