MARCKS Antibody

Images

 
Western Blot: MARCKS Antibody [NB100-82026] - Western blot analysis of extract from starved NIH/3T3 cells, using MARCKS (Ab-158) antibody (Lane 1 and 2) and MARCKS (phospho-Ser158) antibody (Lane 3 and 4).
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NB100-82026] - Staining of methanol-fixed Hela cells using MARCKS (Ab-158) antibody

Product Details

Summary
Product Discontinued
View other related MARCKS Primary Antibodies

Order Details


    • Catalog Number
      NB100-82026
    • Availability
      Product Discontinued

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MARCKS Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around aa. 158 (R-F-S-F-K).
Specificity
MARCKS (specific to a.a. 158) detects endogenous levels of total MARCKS protein.
Clonality
Polyclonal
Host
Rabbit
Gene
MARCKS
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MARCKS Antibody

  • 80K-L protein
  • 80K-L
  • FLJ14368
  • MACS
  • MACSmyristoylated alanine-rich C-kinase substrate
  • MARCKS
  • MRACKS
  • myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
  • myristoylated alanine-rich protein kinase C substrate
  • phosphomyristin
  • PKCSLFLJ90045
  • PRKCSL
  • Protein kinase C substrate, 80 kDa protein, light chain

Background

MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MARCKS Antibody (NB100-82026). (Showing 1 - 1 of 1 FAQ).

  1. Will NB100-82026 (peptide -affinity purified polyclonal antibody to MARCKS (Ab-158)) recognize only the non-phosphorylated sequence around Ser-158 and not the phosphorylated sequence around Ser-158?
    • The NB100-82026 antibody detects the total level of endogenous MARCKS protein, including both non-phosphorylated and phosphorylated forms.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MARCKS Antibody (NB100-82026)

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Bioinformatics

Gene Symbol MARCKS
Entrez
Uniprot