Marapsin/Pancreasin Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Marapsin/Pancreasin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Marapsin/Pancreasin
Ala23-Lys290
Accession # Q9BQR3
Specificity
Detects human Marapsin/Pancreasin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with recombinant mouse Marapsin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRSS27
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunoprecipitation 25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 2 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
Standard:Recombinant Human Marapsin/Pancreasin (Catalog # 1988-SE)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Marapsin/Pancreasin Antibody [Unconjugated]

  • CAP-2
  • CAPH2
  • channel-activating protease 2
  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.4
  • EC 3.4.21.43
  • Marapsin
  • MPN
  • MPNMarapsin
  • Pancreasin
  • protease, serine 27
  • PRSS27
  • serine protease 27

Background

Marapsin, pancreasin, and channel-activating protease 2 (CAP-2) are different names for the same serine protease that is expressed strongly in the pancreas (1, 2). The protein is synthesized with a signal peptide (amino acid residues 1‑22), a pro peptide (residues 23‑34) and a mature chain (residues 35‑290) corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified. The N-terminal sequencing results indicate that the purified protein is a disulfide bond-linked dimer formed between the pro peptide and the mature chain. The active enzyme has low activity against peptide substrates tested, but high activity against thioester substrates. The peptidase activity is inhibited by 20 mM benzamidine.

  1. Bhagwandin, V.J. et al. (2003) J. Biol. Chem. 278:3363.
  2. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF1988
Species: Hu
Applications: WB, IP, B/N, ELISA(Cap)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1988

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Bioinformatics

Gene Symbol PRSS27
Uniprot