MAPKAPK2 Antibody

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Western Blot: Hexokinase 1 Antibody [NBP1-78020] - Analysis using the HRP conjugate of NBP1-78020. Detection of Lane 1: Hexokinase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Hexokinase Antibody Peroxidase ...read more
Western Blot: Hexokinase 1 Antibody [NBP1-78020] - Unstimulated human HeLa whole cell lysate. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at a 1:2000 dilution in crude extracts prepared from either unstimulated or TNFa ...read more
Western Blot: Hexokinase 1 Antibody [NBP1-78020] - Analysis using the HRP conjugate of NBP1-78020. Detection of Hexokinase 1 in yeast (S cerevisiae) whole cell lysate using HRP conjugated Hexokinase 1 antibody. Image ...read more

Product Details

Summary
Product Discontinued
View other related MAPKAPK2 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-78020
    • Availability
      Product Discontinued

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MAPKAPK2 Antibody Summary

Immunogen
Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to aa 310-325 of rabbit MAPKAPK2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
Specificity
This antibody is directed against rabbit MAPKAP Kinase 2. This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPKAPK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 1:15000
Application Notes
This antibody is suitable for western blotting, ELISA, immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. The antibody detects a single 44 kDa protein in crude extracts prepared either from unstimulated or TNFalpha stimulated human HeLa cell lysates. A 1:15,000 dilution is recommended for ELISA. The researcher should determine optimal titers for other applications.
Publications
Read Publications using
NBP1-78020 in the following applications:

  • WB
    3 publications

Reactivity Notes

Reactive in human, mouse, rat. Yeast reactivity reported in scientific literature (PMID: 21772262)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
0.9 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAPKAPK2 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • MAP kinase-activated protein kinase 2
  • MAPK-activated protein kinase 2
  • MAPKAP kinase 2
  • MAPKAPK2
  • MAPKAPK-2
  • mitogen-activated protein kinase-activated protein kinase 2
  • MK2
  • Rps6kc1

Background

MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The best-characterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNFa at a post-transcriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAPKAPK2 Antibody (NBP1-78020)(3)

We have publications tested in 1 confirmed species: Yeast.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
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Filter By Species
Yeast
(3)
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Showing Publications 1 - 3 of 3.
Publications using NBP1-78020 Applications Species
Lee MV, Topper SE, Hubler SL et al. A dynamic model of proteome changes reveals new roles for transcript alteration in yeast Mol Syst Biol (WB, Yeast)

Details:
This publication used the HRP conjugated form of this antibody (Cat# NB120-20547).
WB Yeast
Yao W, King DA, Beckwith SL et al. The INO80 Complex Requires the Arp5-Ies6 Subcomplex for Chromatin-Remodeling and Metabolic Regulation Mol Cell Biol (WB, Yeast)

Details:
This publication used the HRP conjugated form of this antibody (Cat# NB120-20547).
WB Yeast
Yao W, Beckwith SL, Zheng T et al. Assembly of the Arp5 Subunit Involved in Distinct INO80 Chromatin-Remodeling Activities J Biol Chem (WB, Yeast)

Details:
This publication used the HRP conjugated form of this antibody (Cat# NB120-20547).
WB Yeast

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Bioinformatics

Gene Symbol MAPKAPK2
Entrez