MANEA Antibody

Images

 
Western Blot: MANEA Antibody [NBP1-69613] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.
Western Blot: MANEA Antibody [NBP1-69613] - This Anti-MANEA antibody was used in Western Blot of 293T tissue lysate at a concentration of 1ug/ml.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-69613
    • Availability
      Product Discontinued

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MANEA Antibody Summary

Immunogen
Synthetic peptides corresponding to MANEA(mannosidase, endo-alpha) The peptide sequence was selected from the middle region of MANEA. Peptide sequence KVTFHIEPYSNRDDQNMYKNVKYIIDKYGNHPAFYRYKTKTGNALPMFYV.
Clonality
Polyclonal
Host
Rabbit
Gene
MANEA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against MANEA and was validated on Western blot.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for MANEA Antibody

  • alpha 1,2-endomannosidase
  • DKFZp686D20120
  • EC 3.2.1
  • EC 3.2.1.130
  • ENDO
  • Endo-alpha mannosidase
  • endomannosidase
  • FLJ12838
  • glycoprotein endo-alpha-1,2-mannosidase
  • hEndo
  • mandaselin
  • mannosidase, endo-alpha

Background

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Additional MANEA Products

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Bioinformatics

Gene Symbol MANEA
Uniprot