MAML2 Antibody

Western Blot: MAML2 Antibody [NB100-2127] - Detection of Human and Mouse MAML2 on HeLa whole cell lysate using NB100-2127. MAML2 was also immunoprecipitated by another rabbit anti-MAML2 antibodies and NB100-2126.

Immunohistochemistry-Paraffin: MAML2 Antibody [NB100-2127] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-MAML2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MAML2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1153) of human Mastermind-Like 2 using the numbering given in entry AAK93831.1 (GeneID 84441).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAML2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2000-1:15000
• Application Notes: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAML2 Antibody

• DKFZp686N0150
• KIAA1819MGC176701
• MAM2
• mam-2
• MAM-3
• MAM3MLL-MAML2
• mastermind (Drosophila)-like 2
• mastermind-like 2 (Drosophila)
• mastermind-like protein 2

Background

There are 3 MAML (mastermind-like) proteins in mammals (MAML1-3). MAML2 is a critical coactivator for NOTCH signaling. MAML2 associates with NOTCH and CSL in a ternary complex and functions to activate transcription of HES and hairy-related basic helix-loop-helix transcription factors. MAML2 has been identified in a chromosomal rearrangement and fusion with MECT1 in mucoepidermoid carcinomas, secondary acute myeloid leukemia, hidradenomas, and myelodysplastic syndrome. MECT1 is a CREB regulator, and when fused with the transactivational domain of MAML2, imparts constitutive activation of CREB-dependent transcription that may promote oncogenesis. Alternate names for MAML2 include Mam-2, MAM2, MAM3, MAM-3, MLL-MAML2, MGC176701, DKFZp686N0150, and KIAA1819.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAML2 Antibody (NB100-2127)(1)

Publication using NB100-2127

• Oyama T, Harigaya K, Sasaki N et al. Mastermind-like 1 (MamL1) and mastermind-like 3 (MamL3) are essential for Notch signaling in vivo. Development 2011-12-01 [PMID: 22069191]

Bioinformatics

• Gene Symbol: MAML2
• Entrez:
  • 84441(Human)
  • 270118(Mouse)
• Uniprot:
  • AAK93831.1(Human)