Maleimide Activated R-Phycoerythrin

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Product Details

Summary
Applications IHC, IHC-Fr, IHC-P

Order Details

Maleimide Activated R-Phycoerythrin Summary

Description
Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) group. The reactive maleimide groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.

FeaturesBenefits
Compatible protein thiolation kit availableNo specialist knowledge required
Covalent bondHighly stable conjugates
Maleimide activated labels can be used with a range of biomolecules, including oligonucleotidesFlexible
ScalableEasy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Traditional, well-known conjugation chemistryTrusted method


Maleimide activated labels
Maleimide - RPE
Maleimide - HRP
Maleimide - Alkaline Phosphatase
Maleimide - APC
Maleimide-Streptavidin
Maleimide-Ovalbumin
Maleimide-BSA
Maleimide - KLH


If the biomolecule does not have a free thiol group, it will require activation to generate a free thiol group prior to conjugation with the maleimide label:

1. Protein Thiolation Kit

Designed to facilitate the conjugation of proteins to our maleimide-activated labels. Simply add your protein solution to the lyophilized thiolation reagents to activate your protein. These kits are designed to activate 2mg or 5mg of your protein.

Each thiolation kit is also supplied with a thiolation detection kit provided as a positive test for protein thiolation, giving you the option to confirm the chemistry is working.

2. Thiol Quantification Kit

The thiol quantification assay kit allows you to quantify the amount of thiol/sulfhydryl (-SH) groups in samples of thiolated proteins or antibodies. Quantifying the number of free thiols in a sample can be useful in a number of applications and allows for a more controlled conjugation reaction.

The assay is in a convenient 96-well plate format and contains all the necessary reagents required.
Specificity
Maleimide-activated R-Phycoerythrin is designed to facilitate the conjugation of RPhycoerythrin to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to the R-Phycoerythrin allows conjugation reaction is carried out in the pH range 6.5-7.5, creating a stable thio-ether link.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
Publications
Read Publication using 403-0005.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Supplied as freeze dried formulations
Preservative
No Preservative

Notes

Molecules that do not contain -SH groups can be chemically modified to introduce the required thiol functionality. In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols.

This prodcut is manufacture by Innova Biosciences.

Background

Maledimide and periodate activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) or amine (-NH2) group respectively. The reactive maleimide or periodate groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.

Innova Biosciences activated labels are supplied as stable freeze dried formulations, with all material being rigorously QC tested using thiolated biomolecules to ensure high conjugation efficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Maleimide Activated R-Phycoerythrin (403-0005)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Maleimide Activated R-Phycoerythrin (403-0005). (Showing 1 - 1 of 1 FAQ).

  1. I have purchased and would like to use this product: 403-0005, Maleimide Activated R-phycoerythrin. Can you please tell me the molecular weight of this molecule so I may use it in a defined molar ratio to conjugate my antibody?
    • The maleimide modification will not have any significant impact upon the molecular weight. It is recommended using the 240kDa for any calculations that you need.

Additional Maleimide Activated R-Phycoerythrin Products

Maleimide Activated R-Phycoerythrin 403-0005

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