MAdCAM-1 Antibody (MECA-367) [Biotin]

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Flow Cytometry: MAdCAM-1 Antibody (MECA-367) [Biotin] [NBP1-43500] - Staining of mouse TNF alpha-stimulated bEnd.3 cell line with Anti-Mouse MAdCAM-1 FITC. Appropriate isotype controls were used (open histogram). Total ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43500
    • Availability
      Product Discontinued

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MAdCAM-1 Antibody (MECA-367) [Biotin] Summary

Immunogen
Endothelial cells isolated from BALB/c mesenteric and peripheral lymph nodes
Specificity
Murine MAdCAM-1 (Mr 58-88 kDa)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
MADCAM1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The MECA-367 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions and can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for MAdCAM-1 Antibody (MECA-367) [Biotin]

  • hMAdCAM-1
  • MACAM1
  • MAdCAM1
  • MAdCAM-1
  • mucosal addressin cell adhesion molecule 1
  • mucosal addressin cell adhesion molecule-1
  • mucosal vascular addressin cell adhesion molecule 1

Background

The MECA-367 monoclonal antibody reacts with mouse mucosal vascular addressin-1 (MAdCAM-1), a 50-60 kDa member of the Ig superfamily. This antigen is expressed in mucosal lymphoid tissue and lamina propria and is a counter-receptor for CD62L and CD49d. MAdCAM-1 is involved in homing of subpopulation of memory T cells to the gut lamina propria, and recirculation of nave lymphocytes in Peyers patches and mesenteric lymph nodes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MADCAM1
Entrez