Recombinant Human Macrophage Inflammatory Protein 1 alpha Protein

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SDS-Page: Recombinant Human Macrophage Inflammatory Protein 1 alpha Protein [NBP1-51086] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human Macrophage Inflammatory Protein 1 alpha Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-92 of Human Macrophage Inflammatory Protein 1 alpha

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CCL3
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM Sodium Citrate (pH3.5) containing 10% glycerol
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Macrophage Inflammatory Protein 1 alpha Protein

  • C-C motif chemokine 3
  • chemokine (C-C motif) ligand 3
  • G0S19-1Tonsillar lymphocyte LD78 alpha protein
  • LD78ALPHA
  • Macrophage inflammatory protein 1-alpha
  • MIP1AG0/G1 switch regulatory protein 19-1
  • MIP-1-alphaSIS-beta
  • SCYA3PAT 464.1
  • small inducible cytokine A3 (homologous to mouse Mip-1a)
  • Small-inducible cytokine A3

Background

CCL3, also known as, macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This protein has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1beta and RANTES. It acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner. Recombinant human CCL3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CCL3