M-CSF R/CD115 Antibody (AFS98)

Images

 
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Analysis using the Biotin conjugate of NBP1-43363. Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate ...read more
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells ...read more
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Staining of thioglycolate-induced peritoneal exudate cells (PECs) with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (NBP1-43321) (open histogram) or ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, Flow, IHC, IHC-Fr
Clone
AFS98
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

M-CSF R/CD115 Antibody (AFS98) Summary

Immunogen
The immunogen for this antibody was CD115.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CSF1R
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Blocking/Neutralizing
  • Flow Cytometry < 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Blocking/Neutralizing reported in scientific literature (PMID: 24056963)
Publications
Read Publications using
NBP1-43363 in the following applications:

  • B/N
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for M-CSF R/CD115 Antibody (AFS98)

  • CD115 antigen
  • CD115
  • c-fms
  • colony stimulating factor 1 receptor
  • CSF1R
  • CSF-1-R
  • CSFR
  • EC 2.7.10.1
  • FMS proto-oncogene
  • FMSFIM2
  • macrophage colony stimulating factor I receptor
  • macrophage colony-stimulating factor 1 receptor
  • McDonough feline sarcoma viral (v-fms) oncogene homolog
  • M-CSF R
  • MCSFR
  • M-CSFR
  • Proto-oncogene c-Fms

Background

The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for M-CSF R/CD115 Antibody (NBP1-43363)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: B/N.


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B/N
(1)
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Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43363AF405
Alexa Fluor 488 Labeled NBP1-43363AF488
Alexa Fluor 647 Labeled NBP1-43363AF647
Alexa Fluor 700 Labeled NBP1-43363AF700
Allophycocyanin Labeled NBP1-43363APC
Biotin Labeled NBP1-43592
DyLight 350 Labeled NBP1-43363UV
DyLight 405 Labeled NBP1-43363V
DyLight 488 Labeled NBP1-43363G
DyLight 680 Labeled NBP1-43363FR
DyLight 755 Labeled NBP1-43363IR
PE Labeled NBP1-43363PE
PerCP Labeled NBP1-43363PCP

Additional M-CSF R/CD115 Products

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Bioinformatics

Gene Symbol CSF1R
Entrez
Uniprot