Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (5H1)

Images

 
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (5H1) [NBP1-51642] - Western blot analysis using KDM4A mAb against HEK293 (1) and KDM4A-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (5H1) [NBP1-51642] - Immunofluorescence analysis of NTERA-2 cells using KDM4A mouse mAb (green). Red: Actin filaments ...read more
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (5H1) [NBP1-51642] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human larynx cancer tissues ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
5H1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (5H1) Summary

Immunogen
Purified recombinant fragment of human KDM4A expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDM4A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (5H1)

  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM3A
  • JHDM3AJMJD2
  • JmjC domain-containing histone demethylation protein 3A
  • JMJD2
  • JMJD2A
  • JMJD2Alysine-specific demethylase 4A
  • jumonji domain containing 2
  • jumonji domain containing 2A
  • Jumonji domain-containing protein 2A
  • KDM4A
  • KIAA0677jumonji C domain-containing histone demethylase 3A
  • Lysine (K)specific Demethylase 4A
  • Lysine (K)-specific Demethylase 4A

Background

JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KDM4A
Entrez