Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

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Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-77070] - Analysis of JMJD1A in HepG2 cell lysate with JMJD1A antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-77070] - Immunofluorescence of JMJD1A in Rat Liver cells with JMJD1A antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-77070] - Rat liver tissue with JMJD1A antibody at 5 ug/ml.
Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-77070] - Mouse liver tissue lysate with JMJD1A antibody at (A) 1 and (B) 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human JMJD1A. The immunogen is located within amino acids 50 - 100 of JMJD1A.
Specificity
This antibody will not cross-react with JMJD1B or JMJD1C.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Peptide (NBP1-77070PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM2A
  • JHDM2AJMJD1AJHMD2A
  • JmjC domain-containing histone demethylation protein 2A
  • JMJD1
  • JMJD1A
  • jumonji C domain-containing histone demethylase 2A
  • jumonji domain containing 1
  • jumonji domain containing 1A
  • Jumonji domain-containing protein 1A
  • KDM3A
  • KIAA0742
  • KIAA0742DKFZp686A24246
  • Lysine (K)specific Demethylase 3A
  • Lysine (K)-specific Demethylase 3A
  • lysine-specific demethylase 3A
  • testis-specific protein A
  • TSGA
  • TSGADKFZp686P07111

Background

The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist. This antibody will not cross-react with JMJD1B or JMJD1C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KDM3A
Entrez
Uniprot