LyC6 Antibody (ER-MP20) [PE]

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Flow Cytometry: Ly-6C Antibody (ER-MP20) [PE] [NB100-64204] - Peripheral Mouse Granulocytes stained with Rat anti Mouse Ly6C:RPE

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-64204
    • Availability
      Product Discontinued

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LyC6 Antibody (ER-MP20) [PE] Summary

Immunogen
Balb/c macrophage precursor cell hybrids
Specificity
Ly6C (ER-MP20)
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
LY6C1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody is useful for ELISA, Functional, Flow cytometry / FACS analysis, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS, 1.0% BSA, and 5% Sucrose
Preservative
0.09% Sodium Azide
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 1.0 ml distilled water.

Background

Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. This antibody is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by Flow Cytometry. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. This antibody also detects a wide range of endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-64203

Additional LyC6 Products

LyC6 NB100-64204

Bioinformatics Tool for Ly-6C Antibody (NB100-64204)

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Bioinformatics

Gene Symbol LY6C1