Ly-6G Overexpression Lysate (Native)

Images

 
Western Blot: Ly-6G Overexpression Lysate (Adult Normal) [NBP2-06539] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ly-6G.

Product Details

Summary
Applications WB

Order Details

Ly-6G Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_067069. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Ly-6G, mRNA.
Gene
LY6G

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Ly-6G

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Ly-6G Overexpression Lysate (Native)

  • C6orf24
  • G6C
  • Ly 6G
  • Ly6G
  • Ly6g6c
  • LY6G6D
  • lymphocyte antigen 6 complex locus G
  • Lymphocyte antigen 6 complex locus G6D
  • Lymphocyte antigen 6 complex locus protein G6c
  • Ng 24
  • Ng24

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional Ly-6G Overexpression Lysate (Native) Products

Ly-6G NBP2-06539

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Bioinformatics Tool for Ly-6G Lysate (NBP2-06539)

Discover related pathways, diseases and genes to Ly-6G Lysate (NBP2-06539). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Ly-6G Lysate (NBP2-06539)

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Pathways for Ly-6G Lysate (NBP2-06539)

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Blogs on Ly-6G.

Ly6G - a marker for monocytes, granulocytes and neutrophils
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol LY6G

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