Immunohistochemistry-Paraffin: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone marrow using 1:200 dilution of Lot A-1 of Ly-6G antibody (clone ...read more
Flow Cytometry: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - Analysis using the Allophycocyanin conjugate of NBP2-00441. Staining of C57BL/6 bone marrow cells with 0.125 ug of Rat IgG2b kappa Isotype Control APC (blue ...read more
Immunohistochemistry-Paraffin: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone marrow using 1:100 dilution of Lot E04586-1632 of Ly-6G antibody ...read more
Flow Cytometry: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - Staining of mouse bone marrow with Anti-Mouse Ly-6G (Gr-1) FITC and Anti-Human/Mouse CD45R (B220) PE. Total viable cells were used for analysis.
Flow Cytometry: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - 1 ug/1^6 BALB/c bone marrow cells were stained with either rat IgG2b: FITC (as an isotype control) or rat anti-mouse Ly-6G: FITC. Large cells were then gated ...read more
Flow Cytometry: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - Analysis using the PE conjugate of NBP2-00441. Total BALB/c bone marrow cell suspension was stained with Anti-Mouse Ly-6G (Gr-1) PE. Viable cells were gated on ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein A or G purified
Western Blot 1:100-1:2000
Flow Cytometry 1:10-1:1000
The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The RB6-8C5 antibody has also been reported for use immunoprecipitation, immunoblotting (WB) and immunohistochemical staining. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In vivo assay was reported in scientific literature.
Read Publications using NBP2-00441 in the following applications:
The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPIlinked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Ly-6G/Ly-6C/GR-1 Antibody (NBP2-00441)(9)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, In vivo.