Ly-6G/Ly-6C Antibody (RB6-8C5) [PE]

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Flow Cytometry: Ly-6G Antibody (RB6-8C5) [PE] [NBP2-00440] - Total BALB/c bone marrow cell suspension was stained with Anti-Mouse Ly-6G (Gr-1) PE. Viable cells were gated on lymphoid (R2) and myeloid (R3) populations ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, In vivo
Clone
RB6-8C5
Clonality
Monoclonal
Host
Rat
Conjugate
PE
Concentration
0.2 mg/ml

Ly-6G/Ly-6C Antibody (RB6-8C5) [PE] Summary

Immunogen
Normal mouse bone marrow.
Specificity
Studies indicate that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also reacts with an additional subpopulation of Ly-6C high cells.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
LY6G
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • In vivo assay
Application Notes
The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The RB6-8C5 antibody has also been reported for use immunoprecipitation, immunoblotting (WB) and immunohistochemical staining. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In vivo assay was reported in scientific literature.
Publications
Read Publications using NBP2-00440.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Background

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPIlinked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly-6G/Ly-6C Antibody (NBP2-00440)(8)

We have publications tested in 3 applications: IHC-Fr, IHC-P, In vivo.


Filter By Application
IHC-Fr
(2)
IHC-P
(2)
In vivo
(2)
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Showing Publications 1 - 8 of 8.
Publications using NBP2-00440 Applications Species
Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res;67(9):4346-52. 2007 May 1. [PMID: 17483348] (In vivo) In vivo
Sasmono RT, Ehrnsperger A, Cronau SL, Ravasi T, Kandane R, Hickey MJ, Cook AD, Himes SR, Hamilton JA, Hume DA. Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1. J Leukoc Biol;82(1):111-23. 2007 Jul. [PMID: 17438263]
Fan J, Li Y, Vodovotz Y, Billiar TR, Wilson MA. Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation. Am J Physiol Lung Cell Mol Physiol;290(4):L738-L746. 2006 Apr. [PMID: 16272176] (In vivo) In vivo
Alvarez MJ, Prada F, Salvatierra E, Bravo AI, Lutzky VP, Carbone C, Pitossi FJ, Chuluyan HE, Podhajcer OL. Secreted protein acidic and rich in cysteine produced by human melanoma cells modulates polymorphonuclear leukocyte recruitment and antitumor cytotoxic capacity. Cancer Res;65(12):5123-32. 2005 Jun 15. [PMID: 15958556] (IHC-P) IHC-P
Brown CR, Blaho VA, Loiacono CM. Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2. J Immunol;171(2):893-901. 2003 Jul 15. [PMID: 12847259] (IHC-P) IHC-P
Miura M, Fu X, Zhang QW, Remick DG, Fairchild RL. Neutralization of Gro alpha and macrophage inflammatory protein-2 attenuates renal ischemia/reperfusion injury. Am J Pathol;159(6):2137-45. 2001 Dec. [PMID: 11733364] (IHC-Fr) IHC-Fr
Dunussi-Joannopoulos K, Runyon K, Erickson J, Schaub RG, Hawley RG, Leonard JP. Vaccines with interleukin-12-transduced acute myeloid leukemia cells elicit very potent therapeutic and long-lasting protective immunity. Blood;94(12):4263-73. 1999 Dec 15. [PMID: 10590071] (IHC-Fr) IHC-Fr
Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocytedifferentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol;151(5):2399-408. 1993 Sep 1. [PMID: 8360469]

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FAQs for Ly-6G/Ly-6C Antibody (NBP2-00440). (Showing 1 - 1 of 1 FAQs).

  1. Hello. Regarding the following product, could you let us know the buffer formulation?
    • This antibody is supplied in PBS with 0.09% sodium azide.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol LY6G