Ly-49A/D Antibody (12A8)


Flow Cytometry: Ly-49A/D Antibody (12A8) [NBP2-00300] - Staining of 3-day Mouse IL-2 Recobinant Protein (NBP1-43227)-stimulated mouse splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (NBP1-43321) more

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Ly-49A/D Antibody (12A8) Summary

The immunogen for this antibody is Ly-49A/D.
12A8 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49D.
IgG2a Kappa
Protein A or G purified
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  • Flow Cytometry 1:10-1:1000
Application Notes
This 12A8 antibody has been tested by flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. *Direct conjugates are recommended for flow cytometric analysis. This 12A8 antibody has also been reported for use in and immunoprecipitation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous, may contain carrier protein/stabilizer.
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


The 12A8 antibody reacts with Ly-49A and Ly-49D. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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