Ly-49A/D Antibody (12A8)

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Flow Cytometry: Ly-49A/D Antibody (12A8) [NBP2-00300] - Staining of 3-day Mouse IL-2 Recobinant Protein (NBP1-43227)-stimulated mouse splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (NBP1-43321) ...read more

Product Details

Summary
Product Discontinued
View other related Ly-49A/D Primary Antibodies

Order Details


    • Catalog Number
      NBP2-00300
    • Availability
      Product Discontinued

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Ly-49A/D Antibody (12A8) Summary

Immunogen
The immunogen for this antibody is Ly-49A/D.
Specificity
12A8 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49D.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This 12A8 antibody has been tested by flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. *Direct conjugates are recommended for flow cytometric analysis. This 12A8 antibody has also been reported for use in and immunoprecipitation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Background

The 12A8 antibody reacts with Ly-49A and Ly-49D. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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