Rat Lung Whole Tissue Lysate (Adult Whole Normal)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB820-59711
    • Availability
      Product Discontinued

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Rat Lung Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Rat Normal Tissue: Lung

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40 and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Lung

Type
Tissue
Life Stage
Adult
Tissue
Respiratory
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Lung Lysate (NB820-59711). (Showing 1 - 2 of 2 FAQ).

  1. For NB820-59711 lysate, I'd like to know the extraction procedure. Which buffer has been used? Protease, Phosphotase Inhibitors? And how will the lysate be delivered (which buffer and concentration)?
    • A total protein extraction kit was used for the extraction of the total protein lysate. This kit uses a TM Buffer (HEPES (pH7.9), MgCl2, Kcl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, Sodium OrthoVanadate) and a cocktail of protease inhibitors. The protein is delivered at 5 ug/ul concentration. We have protease inhibitor cocktail in the lysate, but we don't release the specific protease inhibitor information.
  2. Is this lysate derived from perfused tissues?
    • Unfortunately, this lysate is not from perfused tissues.

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