| Applications | WB |
| Description | LumiBlue™ ECL Extended Solutions for Western Blotting LumiBlue™ ECL Extended is a solution with a very high level of signal intensity and is most effective for important blots with low protein levels and dilute antibodies. The solution is sensitive enough to detect approximately 150 fg of protein per band and has a signal duration of up to 18 hours to allow multiple rounds of imaging with more flexible experimental design. The working solution is stable for up to three days after activation, allowing convenient experiments spanning multiple days and minimizing waste. LumiBlue™ ECL Extended is optimized for dilute antibody concentrations and sensitive detection of low abundance protein resulting in sensitive blots using less antibody. Benefits of LumiBlue™ ECL Extended Include: ECL kits are based on the enzymatic oxidation of luminol with peroxide, which produces light, usually through horseradish peroxidase (HRP). This method is employed by most traditional ECL kits. However, LumiBlue™ improves upon standard ECL kits by using a patented next generation electron mediator coupled with a novel acylation catalyst to improve performance (called enhancers). The combination of these unique modifications to the ECL process increases reaction efficiency resulting in increased light intensity and less signal decay. Different LumiBlue™ kits are extensively optimized for specific antibody dilutions and protein levels giving higher intensity, longer signal stability and wider linearity over traditional ECL kits. This allows for more flexible and cost effective experimental design. Linear Response LumiBlue™ ECL solutions are enhanced by a novel acylation catalyst, resulting in a more linear response to HRP levels than conventional ECL solutions. This increases the range of antibody concentrations at which reliable measurement can occur. When appropriate antibodies are used protein levels as low as 50 fg (5×10-14g) per band can be detected with LumiBlue™ ECL Extreme. Three Day Working Solution LumiBlue™ ECL solutions are activated by mixing the provided solutions in a 1:1 ratio. This allows smaller volumes to be prepared with LumiBlue™ than with other solutions that require asymmetric ratios such as 40:1. Convenient volumes can be prepared quickly and easily. Once mixed, the ECL Extra working solution is stable for up to three days, permitting larger batches to be made and used for a number of experiments across multiple days, saving time and ensuring that exactly the same solution is used for each blot. |
| Storage | Store at 4°C. |
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