LTRIN Antibody (1009804) [Unconjugated]

Western blot shows lysates of Aedes aegypti LTRIN peptide. PVDF membrane was probed with 2 µg/mL of Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB10178) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LTRIN at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LTRIN protein was serially diluted 2-fold and captured by Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB10178) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB101781) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: All-Multi
• Applications: WB, ELISA
• Clone: 1009804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

LTRIN Antibody (1009804) [Unconjugated] Summary

• Immunogen: Synthetic Aedes aegypti LTRIN peptide
• Specificity: Detects LTRIN in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LTRIN Antibody (1009804) [Unconjugated]

• LTRIN

Background

Salivary gland protein LTRIN, a 15 Kilodalton protein from mosquito Aedes aegypti, has been shown to facilitate the transmission of Zika virus (ZIKV) probably by interfering with signaling through the lymphotoxin-beta receptor (LT beta R).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.