LRH-1/NR5A2 Antibody

Product Discontinued

LRH-1/NR5A2 Antibody Summary

• Immunogen: Synthetic 16 amino acid peptide from ligand-binding domain of human NR5A2.
• Specificity: Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Xenopus (94%), Turkey (94%), Sheep (94%), Chicken (94%), Chimpanzee (100%), Zebrafish (94%), Fish (94%), Opossum (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR5A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
• Control:

  alpha-Fetoprotein/AFP Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Zebrafish (94%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LRH-1/NR5A2 Antibody

• Alpha-1-fetoprotein transcription factor
• B1-binding factor
• b1-binding factor, hepatocyte transcription factor which activates enhancer IIof hepatitis B virus
• B1F
• B1F2
• fetoprotein-alpha 1 (AFP) transcription factor
• FTFLRH1
• FTZ-F1 beta
• FTZ-F1
• FTZ-F1beta
• HB1F
• hB1F-2
• hB1FCYP7A promoter-binding factor
• Hepatocytic transcription factor
• Liver receptor homolog 1
• liver receptor homolog-1
• LRH1
• LRH-1
• LRH-1CPF
• NR5A2
• nuclear receptor NR5A2
• nuclear receptor subfamily 5 group A member 2
• nuclear receptor subfamily 5, group A, member 2

Background

Alpha1-fetoprotein transcription factor (Ftf), a NR5 fushi tarazu-like receptor, also is known as human B1-binding factor (HB1F), CYP7A promoter-binding factor (CPF), and liver receptor homolog 1 (LRH1). Ftf has been shown to affect the transcription of hepatic genes including alpha fetoprotein encoding gene (AFP), viral hepatitis B enhancer II, and 7-alpha-hydroxylase encoding gene (CYP7A1). Ftf has also been shown to affect hepatic cholesterol metabolism and bile acid synthesis. Ftf has been shown to regulate aromatase activities in adipose tissue, and alterations in Ftf expression and/or activity in adipose tissue could have considerable effects on local estrogen production and breast cancer development. LRH has been shown to expressly strongly in gonadal tissues, and may play an important role in the regulation of gonadal function. Three isoforms of Ftf have been identified: wild type (495 aa), CPF variant 1 (contains a 46-aa insertion in the A/B domain), and CPF variant 2 (contains a 172-aa deletion within the D/E domain). Ftf expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR5A2
• Entrez:
  • 174(Human)
• Uniprot:
  • O00482(Human)