LPAR3/LPA3/EDG-7 Antibody

Images

 
Western Blot: LPAR3/LPA3/EDG-7 Antibody [NBP1-00796] - Extracts from MCF7 cells.
Western Blot: LPAR3/LPA3/EDG-7 Antibody [NBP1-00796] - Analysis of EDG-7 (L148) antibody in extracts from Jurkat cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00796
    • Availability
      Product Discontinued

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LPAR3/LPA3/EDG-7 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 114-160 of Human EDG-7.
Clonality
Polyclonal
Host
Rabbit
Gene
LPAR3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
Western blot (WB) analysis of EDG-7 (L148) pAb in extracts from MCF7 cells.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LPAR3/LPA3/EDG-7 Antibody

  • EDG7
  • Edg-7
  • EDG7FLJ98231
  • GPCR
  • HOFNH30
  • LPA-3
  • LPA3LPA receptor 3
  • LPAR3
  • lysophosphatidic acid G-protein-coupled receptor7,LPA receptor EDG7
  • lysophosphatidic acid receptor 3
  • Lysophosphatidic acid receptor Edg-7
  • RP4-678I3

Background

The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LPAR3
Entrez